US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents
- Location: 3644, Königstein, Brittany, Austria
We stained cell lines with lectins that have been used to recognize the following three different epitopes: (i) WGA recognizes all sialic acids, (ii) MAA recognizes α2,3 sialic acid, and (iii) SNA recognizes α2,6 sialic acid. In this report, we demonstrate that AAV1 and AAV6 use both α2,3 and α2,6 N-linked sialic acids for binding and infection. To analyze if the sialylated receptors of AAV1 and AAV6 are glycoproteins or glycolipids, we treated Cos-7 and Pro-5 cells with 200 μg/ml proteinase K. Here's more information about Supplier of sialic acid powder as Raw Material for cosmetics stop by the web site. Cell viability was assessed immediately after proteinase K treatment, and no cytotoxic effect was observed. Sialic acids are abundant on the surfaces of muscle cells, which may partly explain the high transduction efficiency of AAV1 and AAV6 vectors for this tissue.
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